7.1.U1: DNA structure suggested a mechanism for DNA replication (Oxford Biology Course Companion page 347). - Outline the features of DNA structure that suggested a mechanism for DNA replication.
2.7.U1: The replication of DNA is semiconservative and depends on complementary base pairing (Oxford Biology Course Companion page 111). - Describe the meaning of “semiconservative” in relation to DNA replication. - Explain the role of complementary base pairing in DNA replication.
2.7.S2: Analysis of Meselson and Stahl’s results to obtain support for the theory of semi-conservative replication of DNA (Oxford Biology Course Companion page 113). - Compare dispersive, conservative and semi-conservative replication. - Predict experimental results in the Meselson and Stahl experiment if DNA replication was dispersive, conservative or semi-conservative.
2.7.NOS: Obtaining of evidence for scientific theories- Meselson and Stahl obtained evidence for the semi-conservative replication of DNA (Oxford Biology Course Companion page 112). - Describe the procedure of the Meselson and Stahl experiment. - Explain how the Meselson and Stahl experiment demonstrated semi-conservative DNA replication
2.7.U2: Helicase unwinds the double helix and separates the two strands by breaking hydrogen bonds (Oxford Biology Course Companion page 114). - State why DNA strands must be separated prior to replication. - Outline two functions of helicase. - State the role of the origin of replication in DNA replication. - Contrast the number of origins in prokaryotic cells to the number in eukaryotic cells.
7.1.U4: DNA replication is carried out by a complex system of enzymes (Oxford Biology Course Companion page 349). - Outline the role of the following proteins in DNA replications: helicase, topoisomerase (AKA gyrase), single stranded binding proteins, primase, DNA polymerase III, DNA polymerase I, and DNA ligase.
2.7.U3: DNA polymerase links nucleotides together to form a new strand, using a pre-existing strand as a template (Oxford Biology Course Companion page 115). - Describe the movement of DNA polymerase along the DNA template strand. - Describe the action of DNA polymerase III in pairing nucleotides during DNA replication.
7.1.U5: DNA polymerases can only add nucleotides to the 3’ end of a primer (Oxford Biology Course Companion page 350). - Explain the need for RNA primers in DNA replication. - Explain what is meant by DNA replication occurring in a 5' to 3' direction.
7.1.U3: DNA replication is continuous on the leading strand and discontinuous on the lagging strand (Oxford Biology Course Companion page 349). - Compare replication on the the leading strand and the lagging strand of DNA. - Explain why replication is different on the leading and lagging strands of DNA. - Outline the formation of Okazaki fragments on the lagging strand.
3.5.U2: PCR can be used to amplify small amounts of DNA (Oxford Biology Course Companion page 188). - State the function of the PCR. - Describe the selectivity of the PCR. 2.7.A1: Use of Taq DNA polymerase to produce multiple copies of DNA rapidly by the polymerase chain reaction (PCR) (Oxford Biology Course Companion page 115). - Outline the process of the PCR. - Explain the use of Taq DNA polymerase in the PCR.
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